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microarray hybridisation chamber user guide  (Agilent technologies)


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    Agilent technologies microarray hybridisation chamber user guide
    Microarray Hybridisation Chamber User Guide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+chambers/us11976284-506-7-12
    Average 90 stars, based on 1 article reviews
    microarray hybridisation chamber user guide - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Development and characterization of an in vitro model of colorectal adenocarcinoma with MDR phenotype
    Article Snippet: The labeling and hybridization steps were carried out according to the Agilent protocol (Two‐Color Microarray‐Based Gene Expression Analysis version 5.7), using a two‐color design in which HCT‐8/R cells were contrasted within parental HCT‐8.. .. The labeled samples were hybridized to Agilent Human GE 4 × 44K microarray in Agilent microarray chambers (G2534A) at 65° for 18 h. GE arrays were scanned using a Genepix 4000B microarray scanner at 5‐ μ m resolution (Axon Instruments, Foster City, CA, USA). .. Image analysis and initial quality control were performed using the Agilent Feature Extraction Software v9.5.

    Article Title: Exploring the effects of homeopathic Apis mellifica preparations on human gene expression profiles.
    Article Snippet: Elisabetta Bigagli*, Cristina Luceri, Simonetta Bernardini, Andrea Dei, Angelica Filippini and Piero Dolara Department of Neurofarba, Section of Pharmacology and Toxicology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy SIOMI Research Unit, Via Orti Oricellari 26, 50123 Florence, Italy Department of Chemistry, University of Florence, Via della Lastruccia, 3, 50019 Sesto F.no, Florence, Italy

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Microarray:

    Article Title: Development and characterization of an in vitro model of colorectal adenocarcinoma with MDR phenotype
    Article Snippet: The labeling and hybridization steps were carried out according to the Agilent protocol (Two‐Color Microarray‐Based Gene Expression Analysis version 5.7), using a two‐color design in which HCT‐8/R cells were contrasted within parental HCT‐8.. .. The labeled samples were hybridized to Agilent Human GE 4 × 44K microarray in Agilent microarray chambers (G2534A) at 65° for 18 h. GE arrays were scanned using a Genepix 4000B microarray scanner at 5‐ μ m resolution (Axon Instruments, Foster City, CA, USA). .. Image analysis and initial quality control were performed using the Agilent Feature Extraction Software v9.5.

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Article Title: Effects of Extreme Dilutions of Apis mellifica Preparations on Gene Expression Profiles of Human Cells
    Article Snippet: RNA Spike-In kit Agilent Technologies was used as control to monitor and calibrate the linearity, sensitivity, and accuracy of the microarray workflow. .. The Cy3-labeled samples were hybridized to Agilent Human GE 8 × 60 K Oligo 60-mer microarrays in Agilent microarray chambers (G2534A) at 65°C for 18 hours. .. Finally, the microarrays were washed once with the Agilent Gene Expression wash buffer 1 for 1 minute at room temperature followed by a second wash with preheated Agilent Gene Expression wash buffer 2 (37°C) for 1 minute.

    Article Title: Exploring the effects of homeopathic Apis mellifica preparations on human gene expression profiles.
    Article Snippet: Elisabetta Bigagli*, Cristina Luceri, Simonetta Bernardini, Andrea Dei, Angelica Filippini and Piero Dolara Department of Neurofarba, Section of Pharmacology and Toxicology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy SIOMI Research Unit, Via Orti Oricellari 26, 50123 Florence, Italy Department of Chemistry, University of Florence, Via della Lastruccia, 3, 50019 Sesto F.no, Florence, Italy

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Article Title: High affinity synbodies for influenza
    Article Snippet: .. Influenza solutions (250 uL) at five different concentrations (7.5×108, 1.5×109, 3×109, 7.5×109, 1.5×1010 vp/ml) were incubated in competition with protease inhibited bovine serum (5 mg/mL) in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed as before and probed with 5 nM, 250 uL mouse monoclonal anti-influenza NA (BEI Resources, Cat. No: NR-4540) with same competitor in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed and probed with 5 nM, 250 uL AlexaFluor-647 conjugated anti-mouse secondary antibody (Life Technologies, Cat. No: A21235) for 1 hour at 37° C. All the slides were washed, dried, and scanned on an Agilent Microarray Scanner. ..

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Control:

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Software:

    Article Title: Tisochrysis lutea F&M-M36 Mitigates Risk Factors of Metabolic Syndrome and Promotes Visceral Fat Browning through β3-Adrenergic Receptor/UCP1 Signaling
    Article Snippet: Yields of cRNA and the dye incorporation rate were measured with the NanoPhotometer spectrophotometer (IMPLEN). .. Sample mixture was loaded into the Agilent Rat GE 8 × 60 K v2 Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65° for 18 h. Fluorescent signal intensities were detected using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using the Agilent Feature Extraction 9.5.3.1 software.

    Article Title: Colon fibroblasts from Pirc rats ( F344 / NTac‐ Apc am1137 ) exhibit a proliferative and inflammatory phenotype that could support early stages of colon carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent SurePrint G3 rat GE 8x60K microarrays, in Agilent microarray chambers, at 65°C for 18 h. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 Software on an Agilent DNA Microarray Scanner, at a resolution of 2 μm. .. Data were acquired using Agilent Feature Extraction 9.5.3.1 software and values for control spots and spots that did not meet the quality criteria were flagged.

    Virus:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Protease Inhibitor:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Peptide Microarray:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Incubation:

    Article Title: A Simple Platform for the Rapid Development of Antimicrobials
    Article Snippet: .. Virus solutions were prepared in dilution buffer (5 mg/mL BSA + protease inhibitor), applied to the peptide microarray, and incubated in Agilent microarray chambers for 1 hour at 37 °C. .. Microarrays were washed, probed with 250 μL of the appropriate detection antibody in Agilent microarray chambers for 1 hour at 37 °C, washed and probed with 250 μL of 5 nM AlexaFluor-647 conjugated secondary antibody for 1 hour at 37 °C.

    Article Title: High affinity synbodies for influenza
    Article Snippet: .. Influenza solutions (250 uL) at five different concentrations (7.5×108, 1.5×109, 3×109, 7.5×109, 1.5×1010 vp/ml) were incubated in competition with protease inhibited bovine serum (5 mg/mL) in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed as before and probed with 5 nM, 250 uL mouse monoclonal anti-influenza NA (BEI Resources, Cat. No: NR-4540) with same competitor in Agilent microarray chambers for 1 hour at 37° C. Microarrays were washed and probed with 5 nM, 250 uL AlexaFluor-647 conjugated anti-mouse secondary antibody (Life Technologies, Cat. No: A21235) for 1 hour at 37° C. All the slides were washed, dried, and scanned on an Agilent Microarray Scanner. ..

    Hybridization:

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.

    Gene Expression:

    Article Title: Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc -Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis
    Article Snippet: .. The labeled samples were hybridized to Agilent Rat GE 8x60K Oligo 60-mer microarrays, in Agilent microarray chambers (G2534A) at 65°C for 18 h. After hybridization, the microarray slides were washed by using the Agilent Gene Expression Wash Buffers. .. Fluorescent signal intensities were detected by using the Agilent Scan Control 7.0 software on an Agilent microarray scanner, at a resolution of 2 μ m. Image analysis and initial quality control were performed using Agilent Feature Extraction software v9.5.



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing